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Vaccination with human papillomavirus-18 E1 protein plus α-galactosyl-ceramide induces CD8 + cytotoxic response and impairs the growth of E1-expressing tumors

Vaccination with human papillomavirus-18 E1 protein plus α-galactosyl-ceramide induces CD8 + cytotoxic response and impairs the growth of E1-expressing tumors

Vaccine 37(9): 1219-1228

CD8+ T cell-mediated immune response plays a major role in the clearance of virus-infected cells, including human papillomavirus (HPV). The effective treatment of women with normal cytology but persistent high risk-HPV infection or with low-grade intraepithelial lesions could take advantage of novel strategies based on vaccination with viral immunological targets with a wide spectrum of cross-protection. The helicase E1, expressed early during viral replication in HPV infection, is among the most conserved papillomavirus proteins, which makes it a good vaccine candidate. In the present study, we examined E1-specific CD8+ T cell and NK immune responses in a mouse model with α-galactosylceramide (α-GalCer) as an adjuvant. We found that mice immunized with E1 combined with α-GalCer elicited an E1-specific CD8+ T and NK cell cytotoxic responses, which correlated with growth inhibition of grafted melanoma B16-F0 cells expressing E1, both in prophylactic and therapeutic protocols.

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Accession: 066446880

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PMID: 30704821

DOI: 10.1016/j.vaccine.2018.12.036

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