+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

First Report of Late Blight Caused by Phytophthora infestans Clonal Lineage US-24 on Potato (Solanum tuberosum) in Wisconsin



First Report of Late Blight Caused by Phytophthora infestans Clonal Lineage US-24 on Potato (Solanum tuberosum) in Wisconsin



Plant Disease 97(1): 152



Potato (Solanum tuberosum) crops are grown on over 25,090 ha in Wisconsin annually. Late blight, caused by Phytophthora infestans (Mont.) deBary, is a potentially devastating disease that affects tomato and potato crops in Wisconsin every few years when inoculum is introduced and weather conditions favor disease. Incidence and severity of late blight are highly variable in these few years due to differences in pathogen clonal lineages, their timing and means of introduction, and weather conditions. Prevention of this disease through prophylactic fungicide application can cost producers millions of dollars annually in additional chemical, fuel, and labor expenses. Populations of P. infestans in the U.S. have recently undergone significant genetic change, resulting in isolates with unique clonal lineages and epidemiological characteristics (1). In 2010, late blight epidemics were of low severity in discrete portions of a few fields and were seen exclusively on potato in two counties of central Wisconsin. Symptoms included water-soaked to dark brown circular lesions with pale green haloes accompanied by white fuzzy pathogen sporulation typically on leaf undersides in high humidity conditions. Infected plants were collected by professional crop consultants and submitted to the authors at the University of Wisconsin Vegetable Pathology Laboratory in Madison, Wisconsin. Eight isolates of P. infestans were generated from individual leaf samples, representing separate fields, by removing sporangia from sporulating lesions and placing onto Rye A agar amended with rifampicin and ampicillin. Axenic, single zoospore-derived cultures of isolates were generated from parent cultures and maintained on Rye A agar for further characterization. Mycelium was coenocytic with hyphal diameter of 5 to 8 μm (n = 50). Sporangia were limoniform to ovoid, semi- to fully papillate, caducous, had short pedicels, and were 36.22 × 19.11 μm (height × width; n = 50). The average length-width ratio was 1.91. Allozyme banding patterns at the glucose-6-phosphate isomerase (Gpi) locus indicated a 100/100/111 profile, consistent with the US-24 clonal lineage (3,4). Mating type assays confirmed the isolates to be A1 and intermediate insensitivity to mefenoxam was observed in vitro (4). Genomic DNA was extracted with a phenol:chloroform:isoamyl alcohol solution and restriction fragment length polymorphism (RFLP) analysis was performed using the RG-57 probe on a representative isolate and resulted in banding patterns consistent with US-24 (2,3). Clonal lineages of P. infestans documented in Wisconsin in previous epidemics included US-8 in the mid-1990s and US-1 in the 1970s. The US-24 (A1) clonal lineage was very widespread in the U.S. in 2010 and its presence in Wisconsin in the same year as identification of US-22 (A2) posed great concern for potential sexual recombination, oospore production, and soil persistence. Fortunately, the opposite mating types were separated spatiotemporally. To the best of our knowledge, this is the first report of the P. infestans clonal lineage US-24 causing late blight on potato in Wisconsin. References: (1) K. Deahl. (Abstr.) Phytopathology 100:S161, 2010. (2) S. B. Goodwin et al. Curr. Genet. 22:107, 1992. (3) Hu et al. Plant Dis. 96:1323, 2012. (4) A. C. Seidl and A. J. Gevens. (Abstr.) Phytopathology 101:S162, 2011.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 066462157

Download citation: RISBibTeXText

PMID: 30722290

DOI: 10.1094/pdis-09-12-0825-pdn


Related references

First Report of Late Blight Caused by Phytophthora infestans Clonal Lineage US-22 on Tomato and Potato in Wisconsin. Plant Disease 97(3): 423, 2013

First Report of Late Blight Caused by Phytophthora infestans Clonal Lineage US-23 on Tomato and Potato in Wisconsin, United States. Plant Disease 97(6): 839, 2013

First Report of Late Blight Caused by Phytophthora infestans Clonal Lineage US-23 on Potato in Idaho. Plant Disease 99(3): 417, 2015

First Report of Late Blight Caused by Phytophthora infestans Clonal Lineage US-23 on Tomato and Potato in Atlantic Canada. Plant Disease 98(3): 426, 2014

Yield Response of Potato (Solanum tuberosum L.) Genotypes to Late Blight Caused by Phytophthora infestans in Uganda. American Journal of Potato Research 95(4): 423-434, 2018

Evaluation of fungicides for control of potato late blight, 1981 Potato (Solanum tuberosum Green Mountain), late blight; Phytophthora infestans. Fungicide and nematicide tests results American Phytopathological Society7(37): 75-76, 1982

Control of late blight of potato with foliar fungicide sprays, 1981 Potato (Solanum tuberosum Red Pontiac), late blight; Phytophthora infestans. Fungicide and nematicide tests results American Phytopathological Society7(37): 75, 1982

Fungicide control of late blight of potato, 1981 Potato (Solanum tuberosum Katahdin), late blight; Phytophthora infestans. Fungicide and nematicide tests results American Phytopathological Society7(37): 76-77, 1982

Control of late blight of potatoes with fungicides, 1981 Potato (Solanum tuberosum Kennebec), late blight; Phytophthora infestans. Fungicide and nematicide tests results American Phytopathological Society7(37): 79-80, 1982

Control of potato early and late blights with foliar fungicide sprays, 1981 Potato (Solanum tuberosum Superior), early blight; Alternaria solani, late blight; Phytophthora infestans. Fungicide and nematicide tests results American Phytopathological Society7(37): 80-81, 1982

Evaluation of fungicides for control of potato early and late blights, 1981 Potato (Solanum tuberosum Russet Burbank), early blight; Alternaria solani, late blight; Phytophthora infestans. Fungicide and nematicide tests results American Phytopathological Society7(37): 81, 1982

Recent developments in managing tuber blight of potato (Solanum tuberosum) caused by Phytophthora infestans. Canadian Journal of Plant Pathology-Revue Canadienne de Phytopathologie 31(3): 280-289, 2009

Pre-infectional biochemical basis of resistance to late blight (Phytophthora infestans) in potato (Solanum tuberosum). Indian Journal of Agricultural Sciences 75(7): 454-456, 2005

Interval mapping of quantitative trait loci for resistance to late blight [Phytophthora infestans (Mont.) de Bary], height and maturity in a tetraploid population of potato (Solanum tuberosum subsp. tuberosum). Genetics 168(2): 983-995, 2004

Detection of a quantitative trait locus for both foliage and tuber resistance to late blight [Phytophthora infestans (Mont.) de Bary] on chromosome 4 of a dihaploid potato clone (Solanum tuberosum subsp. tuberosum). Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 113(5): 943-951, 2006