+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Optimization of indole acetic acid production by isolated bacteria from Stevia rebaudiana rhizosphere and its effects on plant growth



Optimization of indole acetic acid production by isolated bacteria from Stevia rebaudiana rhizosphere and its effects on plant growth



Journal, Genetic Engineering and Biotechnology 16(2): 581-586



The ability to synthesize Indole-3-acetic acid (IAA) is widely associated with the plant growth promoting rhizobacteria (PGPR). The present work deals with isolation and characterization of such bacteria from the rhizosphere of medicinal plant Stevia rebaudiana and optimization of IAA production from its isolates. The optimization of IAA production was carried out at different pH and temperature with varied carbon and nitrogen sources of culture media. Out of different isolates obtained, three of them were screened as efficient PGPRs on the basis of different plant growth promoting attributes. Isolates CA1001 and CA2004 showed better production of IAA at pH 9 (91.7 µg ml-1) and at temperature 37 °C (81.7 µg ml-1). Dextrose (1%) was found to be the best carbon source for isolate CA1001 with 104 µg ml-1 IAA production. Isolate CA 2004 showed best production of IAA 36 µg ml-1 and 34 µg ml-1 at 1.5% and 1% Beef extract as nitrogen source respectively. Isolate CA 1001 showed 32 µg ml-1 IAA production at 0.5% nicotinic acid concentration. From the current study, CA1001 and CA2004 emerged as noble alternatives for IAA production further which also resulted in root and shoot biomass generation in crop plants, hence can be further used as bio-inoculants for plant growth promotion.

(PDF emailed within 0-6 h: $19.90)

Accession: 066471419

Download citation: RISBibTeXText

PMID: 30733776

DOI: 10.1016/j.jgeb.2018.09.001


Related references

Production of indole-3-acetic acid during growth of the rhizosphere bacteria of the genus Pseudomonas. Mikrobiologiya 65(6): 813-817, 1996

Production of indole-3-acetic acid by rhizosphere bacteria of the genus Pseudomonas during the growth process. Mikrobiologiia 65(6): 813-817, 1996

Actinomycetes isolated from medicinal plant rhizosphere soils: diversity and screening of antifungal compounds, indole-3-acetic acid and siderophore production. World Journal of Microbiology and Biotechnology 25(4): 649-655, 2009

Indole-3-acetic acid (IAA) production trait, a useful screening to select endophytic and rhizosphere competent bacteria for rice growth promoting agents. Methodsx 2: 72-78, 2015

Production of indole-3-acetic acid, aromatic amino acid aminotransferase activities and plant growth promotion by Pantoea agglomerans rhizosphere isolates. Plant and soil 297(1-2): 1-13, 2007

Screening and optimization of indole-3-acetic acid production and phosphate solubilization from rhizobacteria aimed at improving plant growth. Current Microbiology 62(1): 173-181, 2011

Studies on the new sweetening source plant, Stevia rebaudiana in Korea. II. Effects of fertilizer rates and planting density on dry leaf yields and various agronomic characteristics of Stevia rebaudiana. Research Reports of the Office of Rural Development, Crop, Suwon 22: 138-144, 1980

Indole-3-acetic acid production by plant associated bacteria: potential to alter endogenous IAA content and growth of Triticum aestivum L. New Biotechnology 25: S308-S309, 2009

Optimization of Indole Acetic Acid Production by Pseudomonas putida UB1 and its Effect as Plant Growth-Promoting Rhizobacteria on Mustard Brassica nigra. Agricultural Research 2(3): 215-221, 2013

Production of sweet substances in Stevia rebaudiana. 1. Simple determination of sweet glucosides in Stevia plant by thinlayer chromato-scanner and their accumulation patterns with plant growth. Nihon Sakumotsu Gakkai kiji = Japanese journal of crop science 55(2): 189-195, 1986

Plant Growth Regulator Effects on In Vitro Propagation and Stevioside Production in Stevia rebaudiana Bertoni. Hortscience 51(12): 1573-1580, 2016

Studies on the production of sweet substances in stevia rebaudiana 1. simple determination of sweet glucosides in stevia plant by thin layer chromato scanner and their accumulation patterns with plant growth. Japanese Journal of Crop Science 55(2): 189-195, 1986

Growth of Brassica juncea under chromium stress: influence of siderophores and indole 3 acetic acid producing rhizosphere bacteria. Journal of Environmental Biology 26(4): 693-699, 2006

Studies on the new sweetening source plant stevia (Stevia rebaudiana) in Korea. I. Effects of dates of transplanting, taking cuttings and sowing on the growth characteristics and dry leaf yields. Research Reports of the Office of Rural Development, Crop 21: 171-179, 1979

Effects of shade regimes and planting geometry on growth, yield and quality of the natural sweetener plant stevia Stevia rebaudiana Bertoni in north-western Himalaya. Archives of Agronomy and Soil Science 59(7): 963-979, 2013