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Improved production of doubled haploids by colchicine application to wheat (Triticum aestivum L.) anther culture

Improved production of doubled haploids by colchicine application to wheat (Triticum aestivum L.) anther culture

Plant Cell Reports 17(12): 974-979

The aim of this study was to optimize the in vitro chromosome-doubling procedure in wheat anther culture. Colchicine, at concentrations of 100-5000 mg/l, was added to the induction medium for 1-5 days. Beneficial effects were obtained with concentrations of 100 and 1000 mg/l colchicine. With time, significant reductions in embryo-like structures as well as higher doubling indices were found. Similar results were obtained with the high- and low-responding genotypes. Colchicine (100 mg/l), added 5 and 20 days after inoculation for 1 and 3 days increased the induction response, but this value was reduced when colchicine was added 10 or 15 days after inoculation. The doubling effect was similar to the control, except for a significant increase with the 3-day application 20 days after inoculation. The highest success index was reached when colchicine was added to the culture medium after 20 days.

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Accession: 066474013

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PMID: 30736550

DOI: 10.1007/s002990050520

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