+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The dynamic adaptation of primary human endothelial cells to simulated microgravity



The dynamic adaptation of primary human endothelial cells to simulated microgravity



Faseb Journal 33(5): 5957-5966



Culture of human endothelial cells for 10 d in real microgravity onboard the International Space Station modulated more than 1000 genes, some of which are involved in stress response. On Earth, 24 h after exposure to simulated microgravity, endothelial cells up-regulate heat shock protein (HSP) 70. To capture a broad view of endothelial stress response to gravitational unloading, we cultured primary human endothelial cells for 4 and 10 d in the rotating wall vessel, a U.S. National Aeronautics and Space Administration-developed surrogate system for benchtop microgravity research on Earth. We highlight the crucial role of the early increase of HSP70 because its silencing markedly impairs cell survival. Once HSP70 up-regulation fades away after 4 d of simulated microgravity, a complex and articulated increase of various stress proteins (sirtuin 2, paraoxonase 2, superoxide dismutase 2, p21, HSP27, and phosphorylated HSP27, all endowed with cytoprotective properties) occurs and counterbalances the up-regulation of the pro-oxidant thioredoxin interacting protein (TXNIP). Interestingly, TXNIP was the most overexpressed transcript in endothelial cells after spaceflight. We conclude that HSP70 up-regulation sustains the initial adaptive response of endothelial cells to mechanical unloading and drives them toward the acquisition of a novel phenotype that maintains cell viability and function through the sequential involvement of different stress proteins.-Cazzaniga, A., Locatelli, L., Castiglioni, S., Maier, J. A. M. The dynamic adaptation of primary human endothelial cells to simulated microgravity.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 066541900

Download citation: RISBibTeXText

PMID: 30817172

DOI: 10.1096/fj.201801586rr


Related references

Induction of three-dimensional assembly and increase in apoptosis of human endothelial cells by simulated microgravity: impact of vascular endothelial growth factor. Apoptosis 11(5): 749-764, 2006

Secretome of Cultured Human Endothelial Cells in Simulated Microgravity. Bulletin of Experimental Biology and Medicine 167(1): 35-38, 2019

Transcriptomic changes in human umbilical cord blood endothelial cells under simulated microgravity. Doklady. Biochemistry and Biophysics 472(1): 1-4, 2017

Effects of miR-503-5p on apoptosis of human pulmonary microvascular endothelial cells in simulated microgravity. Journal of Cellular Biochemistry 120(1): 727-737, 2019

Effects of simulated microgravity on primary human NK cells. Astrobiology 13(8): 703-714, 2013

The ICAM-1 expression level determines the susceptibility of human endothelial cells to simulated microgravity. Journal of Cellular Biochemistry 119(3): 2875-2885, 2018

Human Micro- and Macrovascular Endothelial Cells Exposed to Simulated Microgravity Upregulate hsp70. Microgravity Science and Technology 21(1-2): 141-144, 2009

Induction of vascular endothelial phenotype and cellular proliferation from human cord blood stem cells cultured in simulated microgravity. Acta Astronautica 56(9-12): 918-922, 2005

Adaptation of a 2-D Clinostat for Simulated Microgravity Experiments with Adherent Cells. Microgravity Science and Technology 25(3): 153-159, 2013

Modulation of human endothelial cell behaviour in simulated microgravity. Journal of Gravitational Physiology 9(1): P273-P274, 2002

Growth of Endothelial Cells in Space and in Simulated Microgravity - a Comparison on the Secretory Level. Cellular Physiology and Biochemistry 52(5): 1039-1060, 2019

Effects of basic fibroblast growth factor on endothelial cells under conditions of simulated microgravity. Journal of Cellular Biochemistry 104(4): 1324-1341, 2008

Conditioned media from microvascular endothelial cells cultured in simulated microgravity inhibit osteoblast activity. Biomed Research International 2014: 857934, 2014

Key Proteins Involved in Spheroid Formation and Angiogenesis in Endothelial Cells After Long-Term Exposure to Simulated Microgravity. Cellular Physiology and Biochemistry 45(2): 429-445, 2018

Simulated microgravity promotes nitric oxide-supported angiogenesis via the iNOS-cGMP-PKG pathway in macrovascular endothelial cells. Febs Letters 584(15): 3415-3423, 2010