+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Proteomic analysis revealed alterations of the Plasmodium falciparum metabolism following salicylhydroxamic acid exposure



Proteomic analysis revealed alterations of the Plasmodium falciparum metabolism following salicylhydroxamic acid exposure



Research and Reports in Tropical Medicine 2: 109-119



Although human respiratory metabolism is characterized by the mitochondrial electron transport chain, some organisms present a "branched respiratory chain." This branched pathway includes both a classical and an alternative respiratory chain. The latter involves an alternative oxidase. Though the Plasmodium falciparum alternative oxidase is not yet identified, a specific inhibitor of this enzyme, salicylhydroxamic acid (SHAM), showed a drug effect on P. falciparum respiratory function using oxygen consumption measurements. The present study aimed to highlight the metabolic pathways that are affected in P. falciparum following SHAM exposure. A proteomic approach was used to analyze the P. falciparum proteome and determine the metabolic pathways altered following SHAM treatment. To evaluate the SHAM effect on parasite growth, the phenotypic alterations of P. falciparum after SHAM or/and hyperoxia exposure were observed. After SHAM exposure, 26 proteins were significantly deregulated using a fluorescent two dimensional-differential gel electrophoresis. Among these deregulated proteins, some were particularly involved in energetic metabolism. And the combinatory effect of SHAM/hyperoxia seems deleterious for the growth of P. falciparum. Our results indicated that SHAM appears to activate glycolysis and decrease stress defense systems. These data provide a better understanding of parasite biology.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 066598484

Download citation: RISBibTeXText

PMID: 30881184


Related references

Proteomic analysis of Plasmodium falciparum induced alterations in humans from different endemic regions of India to decipher malaria pathogenesis and identify surrogate markers of severity. Journal of Proteomics 127(Pt A): 103-113, 2015

Comprehensive proteomic analysis of zygote and ookinete stages of avian plasmodium reveals orthlogs of the unknown early mosquito stages of Plasmodium falciparum. 2007

Fractionation and identification of proteins by 2-DE and MS: towards a proteomic analysis of Plasmodium falciparum. Proteomics 5(16): 4213-4222, 2005

Functional proteomic analysis of the cysteine proteases of Plasmodium falciparum. Experimental Parasitology 105(1): 10, 2003

Metabolomic analysis of patient plasma yields evidence of plant-like α-linolenic acid metabolism in Plasmodium falciparum. Journal of Infectious Diseases 206(2): 238-248, 2012

Proteomic analysis of Plasmodium falciparum histone deacetylase 1 complex proteins. Experimental Parasitology 198: 7, 2019

Metabolomic Analysis of Patient Plasma Yields Evidence of Plant-Like alpha-Linolenic Acid Metabolism in Plasmodium falciparum. 2012

Comparative analysis at the nucleotide level of the genes encoding the lactate dehydrogenase enzyme of Plasmodium vivax and Plasmodium falciparum Plasmodium vivax ve Plasmodium falciparum un laktat dehidrogenaz enzimini kodlayan genlerinin nukleotid duzeyinde karsilastirmali analizi. Turkiye Parazitoloji Dergisi 293: 149-153, 2005

Comprehensive immuno-proteomic analysis of human antibody responses to Plasmodium falciparum.. 2008

Identification of Plasmodium falciparum antigens by antigenic analysis of genomic and proteomic data. Proceedings of the National Academy of Sciences of the United States of America 100(17): 9952-9957, 2003

Proteomic analysis of Plasmodium falciparum schizonts reveals heparin-binding merozoite proteins. Journal of Proteome Research 12(5): 2185-2193, 2013

Proteomic analysis of zygote and ookinete stages of the avian malaria parasite Plasmodium gallinaceum delineates the homologous proteomes of the lethal human malaria parasite Plasmodium falciparum. Proteomics 8(12): 2492-2499, 2008

Proteomic Identification and Analysis of Arginine-Methylated Proteins of Plasmodium falciparum at Asexual Blood Stages. Journal of Proteome Research 16(2): 368-383, 2017

Comparative proteomic analysis of metabolically labelled proteins from Plasmodium falciparum isolates with different adhesion properties. Malaria Journal 5: 67, 2006

Molecular characterization and phylogenetic analysis of Plasmodium vivax , Plasmodium falciparum , Plasmodium ovale , Plasmodium malariae and Plasmodium cynomolgi. Journal of Parasitic Diseases 41(1): 230-236, 2017