+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

CXCR6 + NK Cells in Human Fetal Liver and Spleen Possess Unique Phenotypic and Functional Capabilities

CXCR6 + NK Cells in Human Fetal Liver and Spleen Possess Unique Phenotypic and Functional Capabilities

Frontiers in Immunology 10: 469

ISSN/ISBN: 1664-3224

PMID: 30941128

DOI: 10.3389/fimmu.2019.00469

Tissue-resident Natural Killer (NK) cells vary in phenotype according to tissue origin, but are typically CD56bright, CXCR6+, and CD69+. NK cells appear very early in fetal development, but little is known about when markers of tissue residency appear during gestation and whether the expression of these markers, most notably the chemokine receptor CXCR6, are associated with differences in functional capability. Using multi-parametric flow cytometry, we interrogated fetal liver and spleen NK cells for the expression of a multitude of extracellular markers associated with NK cell maturation, differentiation, and migration. We analyzed total NK cells from fetal liver and spleen and compared them to their adult liver and spleen counterparts, and peripheral blood (PB) NK. We found that fetal NK cells resemble each other and their adult counterparts more than PB NK. Maturity markers including CD16, CD57, and KIR are lower in fetal NK cells than PB, and markers associated with an immature phenotype are higher in fetal liver and spleen NK cells (NKG2A, CD94, and CD27). However, T-bet/EOMES transcription factor profiles are similar amongst fetal and adult liver and spleen NK cells (T-bet-/EOMES+) but differ from PB NK cells (T-bet+EOMES-). Further, donor-matched fetal liver and spleen NK cells share similar patterns of expression for most markers as a function of gestational age. We also performed functional studies including degranulation, cytotoxicity, and antibody-dependent cellular cytotoxicity (ADCC) assays. Fetal liver and spleen NK cells displayed limited cytotoxic effector function in chromium release assays but produced copious amounts of TNFα and IFNγ, and degranulated efficiently in response to stimulation with PMA/ionomycin. Further, CXCR6+ NK cells in fetal liver and spleen produce more cytokines and degranulate more robustly than their CXCR6- counterparts, even though CXCR6+ NK cells in fetal liver and spleen possess an immature phenotype. Major differences between CXCR6- and + NK cell subsets appear to occur later in development, as a distinct CXCR6+ NK cell phenotype is much more clearly defined in PB. In conclusion, fetal liver and spleen NK cells share similar phenotypes, resemble their adult counterparts, and already possess a distinct CXCR6+ NK cell population with discrete functional capabilities.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 066651657

Download citation: RISBibTeXText

Related references

LEU-1+ (CD5+) B cells. A major lymphoid subpopulation in human fetal spleen: phenotypic and functional studies. The Journal of Immunology (1950) 136(2): 505-510, 1986

Leu-1+ (CD5+) B cells. A major lymphoid subpopulation in human fetal spleen: phenotypic and functional studies. Journal of Immunology 136(2): 505-510, 1986

Phenotypic and in vivo functional characterization of immortalized human fetal liver cells. Scandinavian Journal of Gastroenterology 49(6): 705-714, 2014

Phenotypic and functional analysis of human fetal liver hematopoietic stem cells in culture. Stem Cells and Development 16(2): 281-296, 2007

Phenotypic and functional evidence for the expression of CD4 by hematopoietic stem cells isolated from human fetal liver. Blood 89(4): 1364-1375, 1997

Thymic emigrants isolated by a new method possess unique phenotypic and functional properties. Blood 97(5): 1360-1369, 2001

Expression of a unique epithelial antigen recognized by mAb DAS-1 in intact fetal human liver and isolated fetal liver cells. Hepatology 28(4 Part 2): 523A, 1998

Phenotypic characteristics of lymphoid populations of middle gestation human fetal liver, spleen and thymus. Journal of Reproductive Immunology 25(1): 81-88, 1993

T cells from human skin possess unique functional characteristics and regulation required of a first line host defence system. FASEB Journal 10(6): A1034, 1996

Phenotypic and functional characterization of human fetal liver progenitors. Blood 86(10 Suppl 1): 592A, 1995

Regulation of extra- and intracellular expression of CXCR-1 and CXCR-2 in mast cells, T-lymphocytes and dendritic cells derived from blood and human skin. Allergy 56(Suppl 68): 137, 2001

Erythroid colony formation by fetal rat liver and spleen cells in vitro: inhibition by a low relative molecular mass component of fetal spleen. Development 114(1): 213-219, 1992

Do audiovisual media possess unique teaching capabilities?. Media Asia 9(4): 183-191, 199, 1982

Phenotypic and functional characterization of committed and primitive myeloid and lymphoid hematopoietic precursors in human fetal liver. Experimental Hematology 25(5): 387-394, 1997

Human fetal liver cells induce colonies in spleen of lethally irradiated mice. Journal of Clinical Immunology 17(3): 205-211, 1997