+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Characterization of Enterocin 1146, a Bacteriocin from Enterococcus faecium Inhibitory to Listeria monocytogenes

Characterization of Enterocin 1146, a Bacteriocin from Enterococcus faecium Inhibitory to Listeria monocytogenes

Journal of Food Protection 55(7): 497-502

Enterococcus faecium DPC 1146 produces a bacteriocin, enterocin 1146, which is inhibitory to Listeria monocytogenes . Enterocin 1146 was produced in GM17 and in milk. The bacteriocin was partially purified by ammonium sulfate precipitation. Its molecular weight, estimated by SDS-PAGE, was 3.0 kDa. It could be stored at -20°C without loss of activity, but pH had a marked effect on enterocin 1146, which was more stable at both high (up to 120°C) and low temperatures (4°C) at pH 5 than at pH 7 and 9. The sensitivity of 57 strains belonging to 35 different species was studied using a critical dilution assay. L. monocytogenes and L. innocua were most sensitive; enterocin 1146 had a bactericidal effect on Listeria . Starter and nonstarter lactic acid bacteria (except Lactobacillus sake )were insensitive or relatively resistant to the bacteriocin. Genetic determinants for bacteriocin production and immunity do not appear to be plasmid borne.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 066768884

Download citation: RISBibTeXText

PMID: 31071897

DOI: 10.4315/0362-028x-55.7.497

Related references

Inhibition of Listeria in Buffer, Broth, and Milk by Enterocin 1146, a Bacteriocin Produced by Enterococcus faecium. Journal of Food Protection 55(7): 503-508, 1992

Preminary characterization of enterocin MR1024, a bacteriocin from Enterococcus faecalis inhibitory to Listeria monocytogenes. Abstracts of the General Meeting of the American Society for Microbiology 99: 525, 1999

Optimization of the production of enterocin 1146, a bacteriocin produced by Enterococcus faecium. Progress in biotechnology(9): 229-232, 1994

Heterologous expression of enterocin A, a bacteriocin from Enterococcus faecium, fused to a cellulose-binding domain in Escherichia coli results in a functional protein with inhibitory activity against Listeria. Applied Microbiology and Biotechnology 67(4): 532-538, 2005

Anti-Listeria monocytogenes bacteriocin-like inhibitory substances from Enterococcus faecium UQ31 isolated from artisan Mexican-style cheese. Current Microbiology 51(2): 110-115, 2005

Complete sequence of the enterocin Q-encoding plasmid pCIZ2 from the multiple bacteriocin producer Enterococcus faecium L50 and genetic characterization of enterocin Q production and immunity. Applied and Environmental Microbiology 72(10): 6653-6666, 2006

Enterocin 226NWC, a bacteriocin produced by Enterococcus faecalis 226, active against Listeria monocytogenes. Journal of Applied Bacteriology 74(4): 380-387, 1993

Purification and characterization of enterocin LR/6, a bacteriocin from Enterococcus faecium LR/6. Applied Biochemistry and Biotechnology 160(1): 40-49, 2010

Isolation and characterization of two bacteriocins produced by Enterococcus faecium strains inhibitory to Listeria monocytogenes. International Journal Of Food Microbiology. 24(1-2): 211-225, 1994

Production and characterization of enterocin 900, a bacteriocin produced by Enterococcus faecium BFE 900 from black olives. International Journal of Food Microbiology 29(2-3): 255-270, 1996

Identification, Characterization, and Three-Dimensional Structure of the Novel Circular Bacteriocin, Enterocin NKR-5-3B, from Enterococcus faecium. Biochemistry 54(31): 4863-4876, 2015

Enterocin B, a new bacteriocin from Enterococcus faecium T136 which can act synergistically with enterocin A. Microbiology 143: 2287-2294, 1997

Enhanced Control of Listeria monocytogenes by Enterococcus faecium KE82, a Multiple Enterocin-Producing Strain, in Different Milk Environments. Journal of Food Protection 80(1): 74-85, 2017

Biochemical and genetic characterization of enterocin A from Enterococcus faecium, a new antilisterial bacteriocin in the pediocin family of bacteriocins. Applied and Environmental Microbiology 62(5): 1676-1682, 1996

Biochemical and genetic characterization of enterocin P, a novel sec-dependent bacteriocin from Enterococcus faecium P13 with a broad antimicrobial spectrum. Applied and Environmental Microbiology 63(11): 4321-4330, 1997