Molecular Cloning and Expression of The Early Auxin-Responsive Aux/Iaa Gene Family in Nicotiana tabacum

Dargeviciute, A.; Roux, C.; Decreux, A.; Sitbon, F.; Perrot-Rechenmann, C.

Plant and Cell Physiology 39(10): 993-1002


ISSN/ISBN: 0032-0781
DOI: 10.1093/oxfordjournals.pcp.a029311
Accession: 067475913

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Early auxin-regulated tobacco cDNAs, belonging to the Aux/IAA gene family have been isolated by screening of a cDNA library prepared from auxin-treated suspension-grown etiolated seedlings of Nicotiana tabacum. The probes used were either RT-PCR fragments or an insert resulting from mRNA differential display selection. All of them possessed the structural features which characterize the Aux/IAA gene products. The auxin response of three distinct Nt-iaa subclasses has been characterized in terms of kinetics, dose-response and specificity as several plant hormones and chemicals have been tested for their ability to alter Nt-iaa mRNA accumulation. Differences of auxin responses have been observed between the Nt-iaa analysed, revealing significant differences of regulation. The effect of the protein synthesis inhibitor cycloheximide suggested that Nt-iaa2.3, Nt-iaa4.3 and strictly related genes can be classified as primary auxin-responsive genes and Nt-iaa28 as a late one. The steady-state mRNA level of these Nt-iaa has also been determined in organs of tobacco plants.