Effects of aFgf, bFgf, Tgf1 and Igf-I on odontoblast differentiation in vitro

Martín, A.; Unda, F.-J.; Bègue-Kirn, C.; Ruch, J.-V.; Aréchaga, J.

European Journal of Oral Sciences 106(S1): 117-121


ISSN/ISBN: 0909-8836
DOI: 10.1111/j.1600-0722.1998.tb02162.x
Accession: 067618633

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In this work, we investigated the effects of a Fgf and b Fgf alone or combined with TGFβ1 or IGF-I on odontoblast differentiation. Trypsin-isolated dental papillae from day 17 mandibular first molar were cultured in semisolid-agar medium for 6 d. Our results demonstrated that a FGF, b Fgf or combinations of these promoted cell polarization at the periphery of the dental Papillae. Moreover, simultaneous addition of a Fgf and TGFβ1 to dental Papillae cultures induced both polarization and functional differentiation of odontoblast-like cells, as well as extracellular matrix deposition. Combination of a Fgf or b Fgf with IGF-I caused cell polarization at the surface of dental papillae, but matrix secretion was restricted to a few explants. In the presence of b Fgf and TGFβ1, the explants had pronounced cell elongations but no matrix deposition. These results indicate that a Fgf or b Fgf is not able to induce odontoblast differentiation alone. However, both a Fgf and b Fgf can act synergistically with TGFβ1 and IGF-I to strengthen their inductive effects and promote gradients of cytological and functional changes in odontoblast-like cells.