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Use of pyrazinamidase activity on Mycobacterium tuberculosis as a rapid method for determination of pyrazinamide susceptibility

McClatchy, J.K.; Tsang, A.Y.; Cernich, M.S.

Antimicrobial Agents and Chemotherapy 20(4): 556-557

1981


ISSN/ISBN: 0066-4804
DOI: 10.1128/aac.20.4.556
Accession: 067645012

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Pyrazinamidase activity in clinical isolates of Mycobacterium tuberculosis has been previously found to correlate with susceptibility to the antituberculosis drug pyrazinamide. The Wayne method for determining pyrazinamidase activity, a technique also utilized as an aid in identification of mycobacteria, and thin-layer chromatography method were found to be useful screening methods for susceptibility testing, since resistant strains are pyrazinamidase negative. These simple methods overcome the difficulty in growing M. tuberculosis at p H 5.5, as is required in the conventional method of susceptibility testing.

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