Use of pyrazinamidase activity on Mycobacterium tuberculosis as a rapid method for determination of pyrazinamide susceptibility
McClatchy, J.K.; Tsang, A.Y.; Cernich, M.S.
Antimicrobial Agents and Chemotherapy 20(4): 556-557
ISSN/ISBN: 0066-4804 DOI: 10.1128/aac.20.4.556
Pyrazinamidase activity in clinical isolates of Mycobacterium tuberculosis has been previously found to correlate with susceptibility to the antituberculosis drug pyrazinamide. The Wayne method for determining pyrazinamidase activity, a technique also utilized as an aid in identification of mycobacteria, and thin-layer chromatography method were found to be useful screening methods for susceptibility testing, since resistant strains are pyrazinamidase negative. These simple methods overcome the difficulty in growing M. tuberculosis at p H 5.5, as is required in the conventional method of susceptibility testing.