Polymorphism in Metarhizium anisopliae var. anisopliae (Hypocreales: Clavicipitaceae) based on internal transcribed spacer-Rflp, Issr and intron markers

Tiago, P.V.; Carneiro-Leão, M.P.; Lima, M.L.A.; Oliveira, N.T.; Luna-Alves Lima, E.A.

Genetics and Molecular Research 10(3): 1565-1575


ISSN/ISBN: 1676-5680
DOI: 10.4238/vol10-3gmr1069
Accession: 068497362

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Isolates of entomopathogenic fungus Metarhizium anisopliae var. anisopliae were characterized using internal transcribed spacer-RFLP, ISSR and intron splice site primers. Thirty-seven isolates were studied, most of which were obtained from the sugar cane pest, Mahanarva fimbriolata (Hemiptera: Cercopidae) from Tangará da Serra, Southwest Mato Grosso State, Brazil. Internal transcribed spacer-RFLP did not differentiate the isolates of M. anisopliae var. anisopliae, while ISSR and intron primers identified three distinct groups. Variability among these groups was 96% for (GTG)(5) and 100% for the other primers. We found considerable genetic variability, even among isolates from the same geographical origin and host.