Short Communication Identification of a Dna methylation point in the promoter region of the bovine Cyp21 gene

da Silva, A.M.; de Freitas, M.A.R.; Rios, A.F.L.; Renzi, A.; Lôbo, R.B.; Galerani, M.A.V.; Vila, R.A.; Ramos, E.S.

Genetics and Molecular Research 10(3): 1409-1415


ISSN/ISBN: 1676-5680
DOI: 10.4238/vol10-3gmr1266
Accession: 068497372

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The CYP21 (steroid 21-hydroxylase) gene is involved in the synthesis of steroid hormones. Bov-A2 is a retroposon that is common in ruminant genomes. The promoter region of bovine CYP21 contains a short interspersed nucleotide element of Bov-A2, which overlaps a putative Sp1 binding site. We looked for RFLP/HpaII polymorphism in the Bov-A2 element in bovine Zebu breeds by PCR-RFLP, and examined whether polymorphism in this element is associated with methylation. Among DNA samples from 135 Brazilian Zebu breed cattle, we identified an RFLP/HpaII polymorphism (T/C), which, based on a restriction methylation-sensitive assay employing HpaII and isoschizomer MspI enzymes (methylation-sensitive and -non-sensitive enzymes, respectively), appears to be a DNA methylation point. This is the first report of this polymorphism and on DNA methylation in the bovine CYP21 promoter region in Brazilian Zebu cattle.