A quantitative assay of covalently bound cephalosporin derivatives in cephalosporin-protein conjugates

Ebata, M.; Miyake, Y.; Uchida, J.

The Journal of Antibiotics 29(6): 665-666

1976


DOI: 10.7164/antibiotics.29.665
Accession: 068515732

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Abstract
An alternative method to determine covalently bound cephalosporin derivatives quantitatively in cephalosporin-protein conjugates is presented. This method includes a deamination reaction of the free .epsilon.-amino groups of lysine residues by nitrite in acidic medium.