Characterization and developmental regulation of beta-galactosidase isozymes in Dictyostelium discoideum

Dimond, R.L.; Mayer, M.; Loomis, W.F.

Developmental Biology 52(1): 74-82


ISSN/ISBN: 0012-1606
PMID: 9321
DOI: 10.1016/0012-1606(76)90008-7
Accession: 068517856

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Two isozymes of .beta.-galactosidase (EC were identified during growth and development of the cellular slime mold, D. discoideum. The isozymes were partially purified and differ in a variety of physical and enzymatic properties. .beta.-Galactosidase-1 is present in vegetative cells. The specific activity is reduced during early development and then increases again during culmination. The specific activity of .beta.-galactosidase-2 increases in early development and then again during culmination and spore maturation. The specific activity of .beta.-galactosidase-2 is extremely dependent upon growth conditions and is regulated over a 160-fold range. The accumulation of both isozymes is dependent on concomitant RNA and protein synthesis.