The biosynthesis of phosphorylated tyrosine hydroxylase by organ cultures of rat medulla and superior cervical ganglia

Letendre, C.H.; MacDonnell, P.C.; Guroff, G.

Biochemical and Biophysical Research Communications 74(3): 891-897


ISSN/ISBN: 0006-291X
PMID: 14638
DOI: 10.1016/0006-291x(77)91602-3
Accession: 068517976

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Tyrosine hydroxylase was synthesized in organ cultures of rat adrenal medulla and superior cervical ganglia and isolated by immunoprecipitation followed by sodium dodecylsulfate-polyacrylamide gel electrophoresis. When the cultures were grown in radioactive phosphate the tyrosine hydroxylase contained radioactivity. Superior cervical ganglia from animals injected with nerve growth factor made more tyrosine hydroxylase and proportionately more phosphate was incorporated into the enzyme than in ganglia from control animals.