Mechanism of chemiluminescence from the linoleate--lipoxygenase system

Nakano, M.; Sugioka, K.

Archives of Biochemistry and Biophysics 181(2): 371-383

1977


ISSN/ISBN: 0003-9861
PMID: 20046
DOI: 10.1016/0003-9861(77)90242-9
Accession: 068518167

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Abstract
Blue luminescence peaking at 420 nm arises in the early stage of lipoxygenase -catalyzed linoleate oxygenation. An excited species which involves the blue light, excited CO2, is produced by the interaction of an oxidant and carbonate present in the system. An oxidant generated in a linoleate-lipoxygenase system attacks carbonate and also proteins and oxidizable xanthene dyes to produce their electronically excited states, which emit light in the visible region during their return to ground states. This also attacks diphenylisobenzofuran (a singlet oxygen trap) yielding o-dibenzoylbenzene identical with that obtained by a singlet oxygen-derived reaction. Neither an active form of lipoxygenase nor a linoleate peroxy radical is considered to be the oxidant. Another luminescence, which was not characterized spectrometrically, begins to appear when most of the O2 in the system was consumed during the reaction. An excited species, probably involved in this luminescence, can transfer its energy to the dyes containing heavy atoms and is reasonably considered to be an excited carbonyl generated from linoleate peroxy radicals via a cyclic intermediate.