3H]Haloperidol and [3H]spiroperidol binding in rat striatum during ageing

Govoni, S.; Spano, P.F.; Trabucchi, M.

Journal of Pharmacy and Pharmacology 30(7): 448-449

1978


ISSN/ISBN: 0022-3573
PMID: 27613
DOI: 10.1111/j.2042-7158.1978.tb13284.x
Accession: 068518416

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Abstract
Experimental evidence suggests that neurotransmitters in the brain are modified during aging; the synaptic mechanisms of catecholamine, particularly dopaminergic systems, seem to be most affected. To investigate dopaminergic receptor function during aging the technique of labeling postsynaptic dopamine receptor sites with [3H]haloperidol was used. The results, when presented as Scatchard plots of the specific binding in the striatum of mature and senescent rats, showed that the decreased binding by senescent rats of [3H]haloperidol appears to be confirmed with [3H]spiroperidol. This appears to be due to a decreased receptor affinity rather than to the total number of binding sites. The apparent dissociation constant, KD, for [3H]spiroperidol binding is much larger (0.63 mM) in the striatum of 30 mo. than in the 10 mo. animals (0.12 nM). These results clearly support the hypothesis of a decreased dopaminergic receptor affinity with aging.