Changes in HL-A antigen profiles on SV40-transformed human fibroblasts

Pellegrino, M.A.; Ferrone, S.; Brautbar, C.; Hayflick, L.

Experimental Cell Research 97(2): 340-345


ISSN/ISBN: 0014-4827
PMID: 55364
DOI: 10.1016/0014-4827(76)90625-x
Accession: 068518892

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The histocompatibility antigen profile of human fibroblasts transformed with SV40 virus was investigated by determining their ability to specifically absorb HL-A alloantisera. Four out of 4 human adult skin fibroblast lines acquired, after SV40 transformation, the ability to absorb the HL-A alloantiserum VICTOR directed against the specificities HL-A5, W5. On the contrary, none of 6 embryonic fibroblast lines showed any qualitative or quantitative change of their HL-A antigenic profile. Similarly, murine, monkey and hamster cells could not absorb the activity of the HL-A alloantiserum VICTOR after SV40 transformation. The new HL-A antigen specificity on human fibroblasts after SV40 transformation may be due to either a cross-reaction between HL-A specificities and antigenic structures present on glycoprotein(s) coded by the viral genome and expressed on the cell or to a change in the regulating mechanism of the expression of histocompatibility antigens on the cell surface, should the genetic information for all the HL-A specificities be present in the cell genome.