Lindane induction of liver microsomal monoxygenases in rats: effects of a low-calcium diet

Pélisier, M.A.; Faudemay, F.; Manchon, P.; Albrecht, R.

Food and Cosmetics Toxicology 16(1): 27-31

1978


ISSN/ISBN: 0015-6264
PMID: 75826
DOI: 10.1016/s0015-6264(78)80323-x
Accession: 068519579

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Abstract
Two groups of young male rats were fed either a diet with a normal Ca content (1%) or a low-Ca (0.06%) diet for 6 wk. After the first 2 wk, each group was subdivided equally into a control group and a group treated for the remaining 4 wk with 60 ppm lindane in the diet. The rats were then killed, livers and kidneys were removed and weighed and hepatic microsomal fractions were prepared for protein, phosphatidylcholine and enzyme measurements. Lindane increased the kidney weights and the rates of aminopyrine and aniline metabolism in the liver, and induced the NADPH-dependent microsomal electron-transport system (cytochrome P-450, cytochrome c reductase, phosphatidylcholine). Under the experimental conditions used, the low-Ca diet did not appear to affect the metabolism of exogenous substances, although it lowered the P-450 content of the liver microsomes. The maintenance of the normal level of metabolism may be explained by an increase in electron transfer towards the cytochrome from NADPH with induction of NADPH-cytochrome c reductase and NADPH-oxidase. Lindane treatment prevented the characteristic inducing effect of Ca deficiency on the specific molecular activity of NADPH-cytochrome c reductase [reduction of P-450 (Fe3+)].