Studies on the influence of the methanol extraction residue tubercle bacillus fraction on immunological responsiveness of mice. Effects on antibody formation to a soluble protein and a T-independent antigen, delayed hypersensitivity to sheep erythrocytes and numbers of antigen-reactive lymphoid cells

Jacobs, D.M.; Pass, E.; Abraham, C.; Weiss, D.W.

Israel Journal of Medical Sciences 14(1): 60-74


ISSN/ISBN: 0021-2180
PMID: 75868
Accession: 068519580

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In further studies of the immunomodulatory activity of the methanol extraction residue fraction of tubercle bacilli (MER), the effects of MER (in mice) on the following parameters was examined: antibody formation to a soluble protein, bovine .gamma.-globulin (BGG); clearance of BGG from the circulation; formation of specifically induced antigen-reactive cells (ARC) possessing and devoid of, the .theta.-antigen (Thy 1.2); 4) delayed hypersensitivity to sheep red blood cells (SRBC); and the plaque-forming cell (PFC) response to the thymus-independent antigen, trinitrophenylated lipopolysaccharide (TNP-LPS). Adult animals which do not mount a primary antibody response to soluble BGG did produce such antibody after pretreatment with MER and subsequent immunization with 100 .mu.g to 10 mg of the antigen . Animals given MER in the 1st wk of life also responded to BGG immunization at 5 wk of age; and, when treated with MER and primed with BGG during the 1st wk, showed a higher response than the nontreated immunized controls to a later secondary sensitization. Determination of clearance rate and organ localization of 125I-labeled BGG indicated that the antigen is catabolized more rapidly in the 1st hours after introduction. Pretreatment with MER heightened the ability of the animals was increased moderately. The delayed hypersensitivity response to SRBC was elevated in animals given MER previously, especially when the specific immunization stimulus was limited. In contrast to the appreciable stimulatory action of MER on T [thymus-derived] cell-dependent or T cell-mediated reactions, the agent exerted only a slight influence on reactivity to TNP-LPS; the numbers of PFC were increased moderately only at the highest dosage of antigen tested.