Developmental biology of catfish Heteropneustes fossilis Bloch: changing pattern of RNA synthesis and RNA polymerases during embryonic development

Chaudhuri, A.; Sarkar, P.K.; Mandal, R.K.

Indian Journal of Experimental Biology 17(10): 1016-1021


ISSN/ISBN: 0019-5189
PMID: 94902
Accession: 068520600

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The changing pattern of RNA synthesis was studied in developing H. fossilis embryos. The RNA synthesis rate is small but detectable at cleavage stage. This rate continuously increased as development progressed. The nature of the RNA labeled for 1 h at blastula and gastrula stages was examined by polyacrylamide gel electrophoresis. The RNA synthesized at blastula was heterogeneous in size ranging from 4S to over 28S, while at gastrula, much of the RNA was 28S and 18S ribosomal RNA. Fractionation of multiple RNA polymerases by DEAE-cellulose chromatography revealed some changes in the relative proportions of nucleolar RNA polymerase at gastrula, but this enzyme was present in blastula stage as well. The control of ribosomal RNA synthesis is not at the level of the specific RNA polymerase. This control might be at structural level of chromatin, as indicated by a change in its template activity for in vitro RNA synthesis using Escherichia coli RNA polymerase.