Glutamate decarboxylase activity in striatal slices: characterization of the increase following depolarization

Gold, B.I.; Roth, R.H.

Journal of Neurochemistry 32(3): 883-888

1979


ISSN/ISBN: 0022-3042
PMID: 107276
DOI: 10.1111/j.1471-4159.1979.tb04572.x
Accession: 068520853

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Abstract
The activity of glutamate decarboxylase (GAD) in rat striatal slices was estimated following preincubation in either a non-depolarizing medium or in a depolarizing medium. GAD activity was significantly increased following preincubation in normal Krebs-Ringer-phosphate medium as compared to activity in slices which were not preincubated. GAD activity in slices which were depolarized in high K Krebs-Ringer-phosphate medium was further increased when compared to activity in slices which were preincubated in normal medium. The depolarization-induced increase in GAD activity was graded in response to the time of depolarization, and both the increase following preincubation in normal medium and the increase following preincubation in high K displayed a relative requirement for Ca. Net GABA formation from endogenous glutamate was increased in slices preincubated in high K medium as compared to net GABA formation from endogenous glutamate in slices preincubated in non-depolarizing medium. In support of the use of [14C]CO2 trapping as an estimate of GAD activity in slices, preincubation of slices in the presence of the GAD inhibitor glutamic acid .gamma.-hydrazide caused a concentration-related inhibition of [14C]CO2 development.