Characterization of a CV-1 cell cycle. III. Biophysical parameters

Zucker, R.M.; D'Alisa, R.M.; Gershey, E.L.

Experimental Cell Research 122(1): 1-8


ISSN/ISBN: 0014-4827
PMID: 113229
DOI: 10.1016/0014-4827(79)90553-6
Accession: 068520989

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Electronic volume analysis, buoyant density centrifugation and sedimentation velocity were used to measure cellular and nuclear size and density changes during the cell cycle of synchronous [African green monkey kidney] CV-1 cell populations. Similar measurements were made in confluent and random, logarithmically growing CV-1 cell populations. Cellular volume increased linearly throughout the cell cycle while nuclear volume increased in a biphasic manner. Cell density was greatest in G1 phase, decreased in late G1 and early S phases and then increased in late S and G2 phases. Stationary cells had size and density profiles typical of G1 cells while random, logarithmically growing cell populations had size and density profiles typical of mid-S phase cells. Statistical parameters of skewness and coefficient of variation remained constant throughout the cell cycle, indicating that synchronous CV-1 populations moved through the cell cycle as homogenous cell populations with a dispersion of .apprx. 3 h.