Monoclonal IgM, IgA and IgG in the serum of a single individual: immunofluorescence identification of cells producing the immunoglobulins

Krueger, R.G.; Fair, D.S.; Kyle, R.A.

European Journal of Immunology 9(8): 602-606


ISSN/ISBN: 0014-2980
PMID: 115699
DOI: 10.1002/eji.1830090806
Accession: 068521049

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Monoclonal IgM (.vkappa.) and IgA (.vkappa.) in the serum of patient CM were previously shown to share identical, individually specific (Ind) or idiotypic antigenic determinants. A component in the IgG fraction of CM's serum was shown by one assay system to also share Ind determinants with the IgA and IgM. Recent experiments had indicated that the CM IgG monoclonal protein also shares identical Ind determinants with the other 2 CM Ig. The variable regions of H and L chains of the 3 different classes of Ig were apparently very similar. A direct immunofluorescence assay was used in this study on bone marrow smears of patient CM obtained at 2 different dates to identify cells producing these Ig. The reaction of antisera specific for Ind determinants demonstrated that most plasma cells, whether containing IgM, IgA or IgG, stained with the anti-Ind antisera; this occurred irrespective of whether the anti-Ind antisera were generated to the patient's IgM or IgA. The reaction with isotypic antisera revealed cell populations containing IgM, IgA or IgG and a significant cell population containing IgM and IgA. The common occurrence of IgM and IgA-containing cells which synthesize monoclonal Ig with shared Ind determinants provides evidence consistent with the IgM-IgA pathway of differentiation of antibody-producing cells. The tendency toward decreasing numbers of IgM-staining cells with concomitant increasing numbers of IgA and IgG-containing cells suggests that the clones of cells producing these Ig were derived from a single precursor cell.