Effect of guanine nucleotides on the assembly of brain microtubles: ability of 5'-guanylyl imidodiphosphate to replace GTB in promoting the polymerization of microtubules in vitro

Arai, T.; Kaziro, Y.

Biochemical and Biophysical Research Communications 69(2): 369-376

1976


ISSN/ISBN: 0006-291X
PMID: 131550
DOI: 10.1016/0006-291x(76)90531-3
Accession: 068521467

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Abstract
Polymerization of microtubule requires the presence of GTP, and the tubulin-bound GTP is hydrolyzed during microtubule formation. An unhydrolyzable analog of GTP, 5'-guanylyl imidodiphosphate (Gpp(NH)p), was able to replace GTP. The hydrolysis of the terminal phosphate group of GTP does not seem to be a prerequisite to in vitro assembly of microtubules. The microtubules formed in the presence of Gpp(NH)p were indistinguishable from those formed in the presence of GTP under EM, but a remarkable decrease was noted in their sensitivity to depolymerization by Ca2+.