Microtechnique for the stimulation of human lymphocytes by PHA and lymphoblastic cell line (RAJI)

Fague, R.A.; Bohdanowicz, T.; Combes, D.J.; Ilbery, P.L.

Australasian Radiology 21(1): 90-93


ISSN/ISBN: 0004-8461
PMID: 143272
DOI: 10.1111/j.1440-1673.1977.tb02949.x
Accession: 068521953

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The effects of radiotherapy and chemotherapy upon circulatory lymphocytes have been measured by 2 independent means, one monitoring the level of cytogenetic damage appearing in these cells, the other monitoring changes in their proliferative response to stimulation in culture by mitogens. Lymphocyte replicating ability (LRA) can be in response to phytohemagglutin (PHA) or in response to other cells such as in the mixed lymphocyte reaction (MLR). Varying degrees of depression of LRA are being observed within groups of patients undergoing a wide range of cancer therapeutic procedures. Tests on the general immune status of patients enable a profile of the host immune response during cancer therapy to be constructed. Micromethods for large scale processing of lymphocytes from such patients were developed and described.