Gingival inflammation assessed by histology, 3H-estrone metabolism and prostaglandin E2 levels

Holmes, L.G.; Elattar, T.M.

Journal of Periodontal Research 12(6): 500-509

1977


ISSN/ISBN: 0022-3484
PMID: 145489
DOI: 10.1111/j.1600-0765.1977.tb00147.x
Accession: 068522059

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Abstract
Human gingival samples were histologically evaluated and placed in 2 groups, 7 samples each. Group 1 was normal gingiva with no or very few inflammatory cells and group 2 was inflamed gingiva with moderately dense accumulation of inflammatory cells in isolated areas, and sparse distribution in other areas. One hundred to 300 mg gingival tissue samples were separately homogenized in 7 ml of 0.1 M potassium phosphate buffer (pH 7.4) and incubated with 1.51 .times. 10-4 .mu.M of 3H-estrone in the presence of NADPH at 37.degree. C for 3 h. Organic solvent extracts of the homogenates were separated by silica gel TLC and the radioactivity incorporated in estrone (E1) and estradiol-17.beta. (E2) zones was extracted with methanol and measured by liquid scintillation spectrometry. The rate of conversion of (E1) to (E2) in normal and inflamed gingiva was 4.4 and 8.3 .times. 10-7 .mu.M/g/min, respectively. Prostaglandin E2 levels in 3 normal and 2 inflamed gingival samples were 37.8 and 448.7 pmole/g, respectively. The significant increase in the biosynthesis of (E2) and PGE2 in inflamed as compared with normal gingiva could be a systemic factor in aggravating gingival inflammation due to the hyperemic effects of these hormones.