Radioimmunoassay of an antibody to phi X 174 DNA.RNA hybrid

Nakazato, H.

Analytical Biochemistry 98(1): 74-80


ISSN/ISBN: 0003-2697
PMID: 161696
DOI: 10.1016/0003-2697(79)90707-3
Accession: 068522919

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A fast and simple radioimmunoassay (RIA) technique was developed for an antibody to DNA .times. RNA hybrid using protein A-bearing Staphylococcus aureus cells as immunoadsorbent and a glass microfiber filter for the separation of free antigen and antibody-antigen complex. A simple method for preparing 3H-labeled DNA .cntdot. RNA hybrid using single-stranded circular DNA of viruses and Escherichia coli RNA polymerase (nucleosidetriphosphate: RNA nucleotidyltransferase, EC is also described. In comparison to a hybrid made of natural sequences, a synthetic homopolymer hybrid poly(A) .cntdot. poly(dT) was a poor competitor for the antibody by this RIA technique.