Fractionation of ascites tumour cells at 1 g: separation of cells in specific stages of the life cycle

Tulp, A.; Welagen, J.J.

European Journal of Cancer 12(7): 519-526


ISSN/ISBN: 0014-2964
PMID: 182500
DOI: 10.1016/0014-2964(76)90157-2
Accession: 068523233

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Novikoff rat hepatoma ascites cells were separated by sedimentation at 1 g, and populations were obtained which were highly enriched with respect to cells in specific stages of the cell cycle. Sedimentation was done using linear Ficoll gradients in a newly developed sedimentation chamber. This procedure yielded populations which were enriched in G1, S and G2 + M leukemia ascites cells. Labeling index, 3H-thymidine incorporation and lactate dehydrogenase activity of the cells were determined. Viability was about 95%. The newly devised sedimentation chamber allows separation of classes of cells in sufficient quantity for chemical determinations.