The relationship of cAMP levels and the rate of pool labelling of cAMP and related bases, nucleosides and nucleotides to the Hela cell cycle

Al-Bader, A.A.; Rao, P.N.; Orengo, A.

Experimental Cell Research 103(1): 47-54

1976


ISSN/ISBN: 0014-4827
PMID: 186289
DOI: 10.1016/0014-4827(76)90239-1
Accession: 068523326

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Abstract
Cellular cadenine. The cAMP levels were maximal in G 1 and minimal in G 2 and mitosis, as previously reported by others. The rate of labeling of the cAMP pools was maximal in G 2 and decreased to a minimum in G 1. The rate of cAMP synthesis is probably highest when pool level is lowest and vice versa. A comparison of cAMP levels and the rate of 5 AMP pool labeling throughout the HeLa cell cycle indicated an inverse relationship. Such a relationship emphasizes the role of the cyclic 3',5'-phosphodiesterase activity during the cell cycle. The kinetics of pool labeling of IMP, ATP and hypoxanthine throughout the cell cycle suggested that the adenylate energy charge fluctuated as a function of the cell cycle. The apparent activation of the adenylate cyclase during G 2 and mitosis as reflected by the increased rate of cAMP pool labeling suggests that the super phosphorylation of H 1 histone during G 2-mitotic transition may be mediated by cAMP-dependent phosphokinases.