Mitogen induction of murine C-type viruses. III. Effect of culture conditions, age, and genotype

Schumann, G.; Moroni, C.

Virology 79(1): 81-87


ISSN/ISBN: 0042-6822
PMID: 194404
DOI: 10.1016/0042-6822(77)90336-1
Accession: 068523568

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Various parameters affecting the induction of endogenous C-type virus from mouse spleen cell cultures by lipopolysaccharide (LPS) [from Escherichia coli], a B[bone marrow-derived]-lymphocyte mitogen, were examined. Virus induction and mitogenicity, as measured by stimulation of intracellular DNA synthesis, showed similar dose-response patterns suggesting that they are linked phenomena. 5-Bromo-2'-deoxyuridine (BrdU) enhances the virus induction by LPS in a dose-dependent way with an optimum concentration of 5 .mu.g/ml. When the cell concentration was varied, optimal conditions for virus release were between 2 .times. 106-5 .times. 106 cells/ml. Age dependence of virus induction was examined by comparing the effects of LPS and BrdU in cultures from old and young BALB/c mice. Cells from old mice released more virus in response to LPS than those from young mice, but additional BrdU treatment caused no enhancement of virus release. The mitogenic effect of LPS was similar in cultures from old and young mice. Spleen cultures from several strains of mice showed differing response patterns. While BALB/c, C57BL/6 and AKR cultures all released virus in response to LPS, additional BrdU treatment enhanced virus release from BALB/c and C57BL/6 but not from AKR cells. Cultures of 129/J mice are stimulated to divide by LPS but they could not be induced to release virus by this drug alone or in combination with additional BrdU. In B lymphocytes stimulated by LPS, expression of endogenous viral genes is a function of age and genotype unlike their mitogenic response to LPS.