Effect of alpha-methyl ornithine on ornithine decarboxylase activity of rat hepatoma cells in culture

McCann, P.P.; Tardif, C.; Duchesne, M.C.; Mamont, P.S.

Biochemical and Biophysical Research Communications 76(3): 893-899

1977


ISSN/ISBN: 0006-291X
PMID: 197931
DOI: 10.1016/0006-291x(77)91585-6
Accession: 068523730

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Abstract
DL-.alpha.-methyl ornithine (.alpha.-MeOrn), a potent competitive inhibitor of ornithine decarboxylase (L-ornithine carboxy-lyase EC 4.1.1.7) (ODC), caused an increase of ODC activity of rat hepatoma cells cultured in the presence of the compound. The effect was seen only when there was newly synthesized ODC present. The .alpha.-MeOrn mediated increase of ODC activity was independent of RNA synthesis and was expressed at a posttranscriptional level. ODC half-life, measured after cycloheximide treatment, was increased by a factor of 3 in the presence of .alpha.-MeOrn, suggesting the compound slowed enzyme degradation perhaps by its ability to enter the active site.