+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Quantitative extraction, separation, and recovery of adenine-derived radioactivity in bases, nucleosides, and nucleotides from blood platelets using PEI-cellulose thin-layer chromatography



Quantitative extraction, separation, and recovery of adenine-derived radioactivity in bases, nucleosides, and nucleotides from blood platelets using PEI-cellulose thin-layer chromatography



Analytical Biochemistry 81(2): 268-282



A method is reported for the separation and recovery of radioactivity in purine compounds, in particular in c[cyclic]AMP derived from [14C]adenine-prelabeled blood platelets [human]. It is based on Randerath's thin-layer ion-exchange chromatograhy on PEI[polyethyleneimine]-cellulose and allows simple, reproducible and quantitative estimation of radioactivity in most adenine-related purine derivatives. Up to 50 .mu.l of neutralized perchloric acid cell extract (supernatant) can be applied without further treatment to the thin layers. The recovery of added radioactivity as 14C-labeled bases, nucleosides, and nucleotides is 98-100%. Recovery of 3H-labeled compounds appears to vary with their charge and position of label, and is generally not as reproducible. One- and two-dimensional techniques are described. The former resolves: ATP, ADP, AMP, cAMP, IMP, adenosine, adenine, inosine and hypoxanthine: the latter: ATP, ADP, cAMP and 2 additional areas containing, respectively, AMP + IMP and the combined bases and nucleosides.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 068523762

Download citation: RISBibTeXText

PMID: 199078

DOI: 10.1016/0003-2697(77)90698-4


Related references

Quantitative extraction separation and recovery of adenine derived radioactivity in bases nucleosides and nucleotides from blood platelets using poly ethylenimine cellulose thin layer chromatography. Analytical Biochemistry 81(2): 268-282, 1977

Thin-layer chromatography of nucleic acid bases, nucleosides, nucleotides and related compounds. 3. Separation of complex mixtures on cellulose layers. Journal of Chromatography 29(1): 126-132, 1967

Thin-layer chromatography of nucleic acid bases, nucleosides, nucleotides and related compounds. IV. Separation on PEI-cellulose layers using gradient elution and direct fluorometry of spots. Journal of Chromatography 29(1): 133-141, 1967

Inst thin layer chromatography of nucleic acid bases nucleosides nucleotides and related compounds iv separation on pei cellulose layers using gradient elution and direct inst fluorometry of spots. Journal Of Chromatography: 133-141, 1967

Separation of nucleotides nucleosides and the various bases by bi dimensional thin layer chromatography part 1. Annali della Facolta di Agraria Universita Cattolica del Sacro Cuore 9(3): 458-462, 1969

The separation and evaluation of puric bases and their nucleosides and nucleotides by thin-layer chromatography. Clinica Chimica Acta; International Journal of Clinical Chemistry 53(1): 69-77, 1974

Separation of purine 3',5'-cyclic nucleotides and nucleosides by thin-layer chromatography on PEI cellulose. Journal of Chromatography 116(2): 465-467, 1976

Separation by thin-layer chromatography of nucleotides from bases and nucleosides in trichloroacetic acid extracts of cells. Journal of Chromatography 229(2): 460-463, 1982

Separation of picomolar concentrations of adenine and guanine nucleotides and nucleosides by high-performance thin-layer chromatography on silica gel. Biochemical Society Transactions 6(5): 1047-1048, 1978

Resolution of complex mixtures of nucleic acid bases, nucleosides, and nucleotides by two-dimensional thin-layer chromatography on polyethyleneimine-cellulose. J Chromatogr 35(4): 531-537, 1968

PEI-cellulose thin-layer chromatography: a highly versatile system for separating purine and pyrimidine nucleotides from nucleosides and free bases. Analytical Biochemistry 50(1): 35-39, 1972

Thin-layer chromatography of nucleic acid bases, nucleosides, nucleotides and related compounds. I. Quantitative analysis by direct fluorometry. Journal of Chromatography 23(3): 465-467, 1966

Thin-layer chromatography of nucleic acid bases, nucleosides, nucleotides and related compounds. IX. Quantitative analysis in situ reflectance spectroscopy. Journal of Chromatography 45(2): 284-290, 1969

The separation of purine and pyrimidine bases and nucleosides by thin-layer chromatography on Sephadex-cellulose and Sephadex-silica gel layers. Journal of Chromatography 70(1): 182-186, 1972

Adenine nucleotide metabolism of human platelets. Thin-layer chromatographic separation of labelled adenine nucleotides. Thrombosis et Diathesis Haemorrhagica 25(2): 223-233, 1971