Hormonal effects on fat cell adenosine 3',5'-monophosphate dependent protein kinase

Guinovart, J.J.; Lawrence, J.C.; Larner, J.

Biochimica et Biophysica Acta 539(2): 181-194


ISSN/ISBN: 0006-3002
PMID: 204358
DOI: 10.1016/0304-4165(78)90005-3
Accession: 068523960

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Fat cell cAMP before electrophoresis, essentially all of the radioactivity was found in Peak III. Peak I probably represents a holoenzyme form and Peak III is compsed of the regulatory subunits of this enzyme. Peak II may represent a relatively inactive holoenzyme form not previously described. Incubation of adipocytes with epinephrine resulted in a dose- and time-dependent decrease in Peak I and increase in Peak III, and insulin opposed these effects of epinephrine. After 1 min incubations with epinephrine, the decreases in Peak I or increases in Peak III correlated with increases in phosphorylase a activity, decreases in glycogen synthase I activity and changes in cAMP, both in the presence and absence of insulin. After incubation with epinephrine for more than 2 min in the presence of insulin, phosphorylase a activity did not correlate with cAMP, suggesting that factors other than the cyclic nucleotide mediate the effects of epinephrine and insulin.