Properties of simian virus 40 and BK virus tumor antigens form productively infected and transformed cells

Simmons, D.T.; Takemoto, K.K.; Martin, M.A.

Virology 85(1): 137-145

1978


ISSN/ISBN: 0042-6822
PMID: 205997
DOI: 10.1016/0042-6822(78)90418-x
Accession: 068524023

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Abstract
SV-40-specific tumor antigen (T Ag) was immunoprecipitated from extracts of productively infected cells and virus-transformed cells and had the same MW of approximately 97,000 as measured by acrylamide gel electrophoresis. In addition to T Ag proteins of that size, lower MW forms were immunoprecipitated with anti-T serum. Experiments using the protease inhibitor L-1-tosylamide-2-phenylethylchloromethyl ketone (TPCK) indicated that nearly all of the smaller SV-40 T Ag polypeptides were artifacts of the extraction procedure. The BK virus-specific T Ag was immunoprecipitated from extracts of infected human [embryo kidney HEK] cells and coelectrophoresed with SV-40 T Ag (97,000 daltons). [African green monkey kidney AGMK cells were also used.].