Rat osteogenic sarcoma cells: comparison of the effects of prostaglandins E1, E2, I2 (prostacyclin) , 6-keto F1alpha and thromboxane B2 on cyclic AMP production and adenylate cyclase activity

Crawford, A.; Atkins, D.; Martin, T.J.

Biochemical and Biophysical Research Communications 82(4): 1195-1201


ISSN/ISBN: 0006-291X
PMID: 212039
DOI: 10.1016/0006-291x(78)90313-3
Accession: 068524250

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The effects of prostaglandins(PG)E1, E2, I2 (prostacyclin), 6-keto F1.alpha. and thromboxane (Tx) B2 were compared in freshly isolated cells from a rat osteogenic sarcoma and in membranes from cultured cells of the same tumor. Cyclic AMP production was measured in cells and adenylate cyclase activity was measured in cell membranes. In both systems PGI2 was less potent than either of the PGE's, and both TxB2 and 6-keto PGF1.alpha. were only weak agonists. These effects on bone-derived cells suggest that PGI2 is unlikely to be a potent bone resorbing agent. Resistance experiments suggested that all the PG's share the same receptor site which appears distinct from the site of action of parathyroid hormone.