Electrode-based immunoassays using urease conjugates

Meyerhoff, M.E.; Rechnitz, G.A.

Analytical Biochemistry 95(2): 483-493

1979


ISSN/ISBN: 0003-2697
PMID: 222166
DOI: 10.1016/0003-2697(79)90760-7
Accession: 068524627

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Abstract
[Jack bean] urease conjugates were employed for competitive-binding enzyme immunoassays (EIA) of a model antigen, bovine serum albumin (BSA), and of cyclic cAMP. Urease activity bound to a double-antibody solid phase was determined with an ammonia gasensing electrode, after appropriate washing steps. Cyclic AMP analogs coupled to urease were used to determine their effect on the overall response characteristics of the cAMP assay. The use of urease as a label for EIA purposes is yielded sensitive assays for both proteins (BSA < 10 ng/ml) and haptens (cAMP < 10-8 M) with good day-to-day reproducibility.