Adenosine diphosphate ribosylation of histone and nonhistone chromosomal proteins with oxidized nicotinamide adenine dinucleotide and 2'-deoxynicotinamide adenine dinucleotide using nuclei isolated from rat liver and Hela cells

Lichtenwalner, D.M.; Suhadolnik, R.J.

Biochemistry 18(17): 3749-3755

1979


ISSN/ISBN: 0006-2960
PMID: 224917
DOI: 10.1021/bi00584a016
Accession: 068524723

Download citation:  
Text
  |  
BibTeX
  |  
RIS

Article/Abstract emailed within 0-6 h
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Abstract
By use of sodium dodecyl sulfate-polyacrylamide and acid-urea gel electrophoresis, evidence is presented that poly(ADP)-ribose polymerase of nuclei isolated from HeLa cells and rat liver preferentially transfers the ADP-ribose moiety from NAD+ to the H1 histones. With nuclei isolated from normal rat liver, the minor H1 histone, H1.degree., is ADP ribosylated. With nuclei isolated from normal rat liver or HeLa cells, poly(ADP-ribose) polymerase preferentially transfers the 2'-dADP-ribose moiety of 2'-deoxyNAD+ to the nonhistone proteins. The average chain lengths of poly(ADP-ribose) from NAD+ covalently bound to either HeLa or rat liver histone proteins are 5 and 7.2, respectively, whereas the average chain length of ADP-ribose associated with the nonhistone proteins of nuclei isolated from rat liver and HeLa cells is 1.7. With 2'dNAD+, the average chain length of the 2'dADP-ribose covalently bound to the histone and nonhistone proteins is 1.2. The difference in the average chain length and the marked differences in the ADP ribosylation of the histone and nonhistone proteins with Nad+ and 2'dNAD+, respectively, demonstrate the importance of the 2'-hydroxyl group of NAD+. Earlier observations that the replacement of the 2'-hydroxyl group of NAD+ with an H atom causes a 10-fold increase in the inhibition of DNA synthesis in nuclei isolated from normal rat liver and restores the ability to inhibit DNA synthesis in nuclei isolated from neoplastic tissue and fetal rat liver (Suhadolnik et al., 1977) are confirmed. This inhibition of DNA synthesis in neoplastic tissue and fetal rat liver is ineffective with NAD+, but is restored by 2'dNAD+.