Stem cell (CFU-C) proliferation and emergence in a case of chronic granulocytic leukaemia: the role of the spleen

Bagby, G.C.

Scandinavian Journal of Haematology 20(3): 193-199


ISSN/ISBN: 0036-553X
PMID: 273978
DOI: 10.1111/j.1600-0609.1978.tb02447.x
Accession: 068525342

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Markedly increased numbers of CFU-C are characteristically found in the peripheral blood of patients with chronic granulocytic leukemia (CGL). The likely sources of these circulating CFU-C are the spleen and/or bone marrow. Using the double layer agar technique, peripheral blood cloning efficiency was measured serially, prior to and after splenectomy in a man with CGL. Cloning efficiencies were measured of splenic venous blood, spleen cell suspensions and marrow cell suspensions on the day of surgery. To estimate the proliferative status of CFU-C, peripheral blood cells as well as spleen and marrow cell suspensions were exposed to cytosine arabinoside for 60 min prior to culture. Peripheral blood cloning efficiency (155 .+-. 8 colonies/2 .times. 105 cells) decreased markedly (10 .+-. 3) after splenectomy. Cloning efficiency of splenic venous blood (288 .+-. 34) was twice that of peripheral venous blood. The surviving fraction of splenic CFU-C after exposure to cytosine arabinoside was 16% compared to survivals of 67 and 66% for marrow and peripheral blood CU-C, respectively. The spleen was primarily responsible for the release of CFU-C into the peripheral blood. The splenic microenvironment may have exerted a more marked stimulatory effect on the replicative activity of CFU-C than the microenvironment of the marrow.