Studies on the recognition of xenogeneic cells by nonimmune macrophages. I. Destruction of chicken fibroblasts in vitro by murine macrophages

Cabilly, S.; Gallily, R.

Cellular Immunology 29(1): 54-65


ISSN/ISBN: 0008-8749
PMID: 300297
DOI: 10.1016/0008-8749(77)90274-x
Accession: 068525595

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Unsensitized murine macrophages are cytotoxic in vitro to chicken embryonic fibroblasts, but not to syngeneic or allogeneic fibroblasts. Stasis and lysis of chicken fibroblasts were observed in mixed macrophage-fibroblast cultures following 24 or 48 h of incubation at effector to target cell ratios of 3:1-8:1. Elucidation of the macrophage xenogeneic killing mechanism clarified the following: unsensitized, unstimulated macrophages lysed chicken fibroblasts as efficiently as thioglycollate-stimulated macrophages; physical contact between effector and target cells is necessary for killing to take place. Cytophilic antibodies do not participate in foreign recognition, as treatment with anti-mouse .gamma.-globulin or trypsin during the incubation period did not diminish macrophage cytotoxicity. Lymphocytes do not seem to participate in macrophage xenogeneic killing in the in vitro system, as macrophage cytotoxicity was not affected by pretreatment of the peritoneal cell population with anti-lymphocyte serum or anti-.theta. serum and complement.