The demonstration of acid alpha-naphthyl acetate esterase activity in human lymphocytes: usefulness as a T-cell marker

Knowles, D.M.; Hoffman, T.; Ferrarini, M.; Kunkel, H.G.

Cellular Immunology 35(1): 112-123


ISSN/ISBN: 0008-8749
PMID: 304379
DOI: 10.1016/0008-8749(78)90131-4
Accession: 068525744

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The histochemical demonstration of nonspecific acid .alpha.-naphthyl acetate esterase (ANAE) activity was evaluated as a T rosettes. Atypical and variable staining patterns were observed in established lymphoid cell lines. The histochemical demonstration of ANAE is simple and reproducible; preparations may be counterstained for cytomorphologic detail and mounted as a permanent record. Certain disadvantages are discussed. The method represents a practical alternative to E rosette assays. It is particularly well suited for certain routine laboratories.