On the mechanism of early recovery of specifically depleted lymphoid cell populations by nonspecific activation of T cells

Prunet, J.; Birrien, J.L.; Panijel, J.; Liacopoulos, P.

Cellular Immunology 37(1): 151-161

1978


ISSN/ISBN: 0008-8749
PMID: 306882
DOI: 10.1016/0008-8749(78)90183-1
Accession: 068525816

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Abstract
Specific depletion from normal CBA mouse spleen cells of those bound on pigeon erythrocyte (PRBC) immunoadsorbent columns before transfer of the depleted population into irradiated syngeneic recipients resulted in elimination of the anti-PRBC responsiveness as assessed by rosette (RFC) and hemolytic plaque (PFC) formation. The anti-sheep erythrocyte (SRBC) responses of cell populations treated in the same manner remained unimpaired. When these populations were stimulated with PRBC and muramyl dipeptide (MDP), an early recovery of specific anti-PRBC responsiveness was produced. PFC response in particular, suddenly increased between the 4th and 5th day after transfer and stimulation thus exhibiting a doubling time of only 4-6 h. This effect of MDP was T[thymus-derived]-cell dependent since treatment of the depleted population with anti-.theta. antigen serum and complement hindered early recovery. Depleted populations stimulated with PRBC alone resumed their T-dependent RFC (but not PFC) responsiveness after the 9th day. In spite of the existence of these educated T cells, a 2nd stimulation on the 10th day with PRBC was unable to elicit a specific PFC response. Stimulation with MDP alone on the day of cell transfer (day 0) followed by stimulation with PRBC on day 10 resulted in a specific PFC response on day 15. MDP appeared to do more than simply promote education of T cells by antigen. In vitro cultures of depleted populations also recovered their specific reactivity when stimulated by antigen and MDP.