Composite activities on B cells of products released by T cells activated by concanavalin a

Primi, D.; Lewis, G.K.; Goodman, J.W.

European Journal of Immunology 9(8): 607-612

1979


ISSN/ISBN: 0014-2980
PMID: 315320
DOI: 10.1002/eji.1830090807
Accession: 068526118

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Abstract
Supernatants from murine spleen cells cultured for 48 h in the presence of 1.0 .mu.g of concanavalin (Con) A induced polyclonal antibody synthesis in cultures of spleen cells from normal and athymic mice in the absence of exogenous antigen. Con A-induced supernatant rescued B [bone marrow-derived] cells which had been rendered unresponsive by a tolerogen [haptenated poly(DGlu,DLys)]. Capacity of the supernatant to induce cell proliferation was studied under high- and low-density culture conditions. In contrast to antibody secretion, proliferation was only detectable in low-density cultures. The Con A-induced supernatant also contained suppressive components, since the primary anti-sheep red blood cell (SRBC) response was markedly suppressed when the antigen added to cultures consisted of SRBC which had previously been used for absorbing the supernatants. Absorbed supernatants displayed enhanced helper activity indicating that only the suppressor component was removable by antigen. The suppressive component was eluted from erythrocytes with ammonium thiocyanate and was itself strongly suppressive when added to cultures with fresh erythrocytes. The suppressive component proved to be highly antigen-specific, as the SRBC-absorbed factor did not affect the response to horse RBC. Supernatants from Con A-activated spleen cells apparently contain helper and suppressor factors, the latter having easily demonstrable antigen specificity. A nonantigen-derived signal is apparently sufficient to trigger proliferation and antibody synthesis by B cells.