Structural changes in the cell membrane of lambda-lysogenic Escherichia coli induced by colicin E2

Yamamoto, H.; Beppu, T.; Arima, K.

Biochemical and Biophysical Research Communications 74(3): 1077-1082


ISSN/ISBN: 0006-291X
PMID: 320975
DOI: 10.1016/0006-291x(77)91628-x
Accession: 068526214

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The structural changes in the cell membrane of .lambda.-lysogenic E. coli induced by colicin E2 were examined. The addition of colicin E2 made the cells susceptible to various detergents and the transport rate of o-nitrophenyl-.beta.-D-galactoside into the colicin-treated cells was stimulated markedly by adding a low concentration of sodium dodecyl sulfate. The fluorescence intensity of 8-anilino-1-naphthalenesulfonate bound to the cells was markedly increased by adding colicin E2. Colicin E2 stimulated the incorporation of 32P from prelabeled phosphatidylglycerol to cardiolipin. The structural alterations of the cell membrane were probably dependent on the presence of the rex gene of .lambda. prophage in the cells.