Evidence for a surface receptor for human migration inhibitory factor on a B-lymphoid cell line

McLeod, T.F.; Cordeiro, P.G.; Meltz, S.K.; Glade, P.R.

Cellular Immunology 37(2): 298-307

1978


ISSN/ISBN: 0008-8749
PMID: 350423
DOI: 10.1016/0008-8749(78)90198-3
Accession: 068526772

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Abstract
Reception by PGLC-33H target cells for the migration inhibitory factor (MIF) produced by this established line was investigated by pulse time and temperature dependence, MIF absorption and abrogation by trypsinization. PGLC-33H supernatants containing MIF were concentrated 5.times. with Carbowax and dialyzed against serum free RPMI-1640 before use. Prior to standard capillary migration assay, a minimum 30 min pulse of MIF at 37.degree. C is required for significant migration inhibition (MI > 20%). No significant MI is observed when cells are pulsed at 4.degree. C for up to 2 h. Preincubation with PGLC-33H for 1 h at 37 C reduces activity of supernatants from 38 to 13% MI; at 4.degree. C to 27% MI. Trypsinization of target cells for 30 min at 25.degree. C abrogates response to MIF (43 to -1 MI). Trypsinized cells did not reduce activity of supernatants. MIF activity is abolished (32 to 3% MI) in samples preincubated with supernatants of the trypsinized cells inactivated with serum. Cells from the human B-lymphoid cell line PGLC-33H have a surface receptor for human MIF.