Anti-immunoglobulin stimulation of murine lymphocytes. IV. Re-expression and fate of cell surface receptors during stimulation

Weiner, H.L.; Scribner, D.J.; Moorhead, J.W.

Journal of Immunology 120(6): 1907-1912


ISSN/ISBN: 0022-1767
PMID: 351062
Accession: 068526790

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Anti-immunoglobulin (anti-Ig) stimulation of spleen cells from aged mice (7-9 mo. old) results in blast cell transformation and increased incorporation of 3H-TdR cells; 85-90% of anti-Ig-induced blast cells, cultured to allow re-expression of surface markers, were positive for surface Ig which was IgM. The prolonged requirement for anti-Ig in culture indicated that surface Ig must be continuously re-expressed by the responding cells. Free surface Ig was not detected on the cells during continuous culture with anti-Ig. The cells has relatively large amounts of rabbit antibody bound to their surface. Similar cultures stimulated with F(ab')2 fragments or cultures of spleen cells from young animals stimulated with intact anti-Ig did not have surface-bound rabbit antibody. The rabbit antibody is most likely in the form of antigen-antibody complexes, i.e., surface Ig-anti-Ig, bound to Fc receptors on the stimulated cells. These complexes are apparently produced by the continuous re-expression and redistribution of surface Ig by anti-Ig, a process that is required for anti-Ig stimulation of B cells.