Studies on the mechanism of lymphocyte-mediated cytolysis. XI. the role of lectin in lectin-dependent cell-mediated cytotoxicity

Green, W.R.; Ballas, Z.K.; Henney, C.S.

Journal of Immunology 121(4): 1566-1572

1978


ISSN/ISBN: 0022-1767
PMID: 359710
Accession: 068526950

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Abstract
The role of lectin in lectin-dependent cell-mediated cytolysis was pursued by using alloimmune C57BL/6 cell mitogens were similarly unable to support lysis even when co-incubated with agglutinating agents. The role of the T cell mitogens in lectin-dependent cytolysis appeared to be 2-fold: activation of the effector cell to express its cytotoxic potential and bridging of the effector cell to its target. Agglutination of target cells to T effector cells, although necessary to elicit cytolysis, was insufficient. T cells activated by mitogen under conditions which precluded cell-cell bridging did not lead to lysis. No evidence was found that the bridging function of the lectin during cytolysis could be served by agents other than T cell mitogens. The Ca2+ ionophore A23187, which substitutes for Con A in the early stages of a mitogenic response but does not cause cell agglutination, did not mediate cytolysis, even in the presence of nonmitogenic agglutinins. Stringent requirements for the activating and bridging roles of lectin during cytolysis necessitate revision of previous notions that the lectins serve merely as a glue.