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Mass fragmentografic determination of moroxydin hydrochloride (ABOB) in blood and urine



Mass fragmentografic determination of moroxydin hydrochloride (ABOB) in blood and urine



Yakugaku Zasshi 99(4): 387-392



A highly sensitive and specific method for the quantitative determination of N1,N1-anhydrobis(.beta.-hydroxyethyl)biguanide (ABOB, moroxydin hydrochloride) in human serum and urine was developed. A triazine derivative derived from ABOB was analyzed by mass fragmentography and a similar triazine derivative from 1-n-butylbiguanide (buformin) was synthesized and used as an internal standard. The molecular ions of ABOB and buformin triazine derivatives, m/e [mass/charge ratio] 249 and 235, respectively, were recorded after elution from the gas chromatographic column, and ABOB in biological fluid was determined. Mass fragmentographic analysis requires less than 5 min and allows an accurate determination of ABOB in the range of 0.02-1 .mu.g/ml of human serum. The standard deviation is less than 12% in the 0.1-1 .mu.g/ml range. A time course study of blood concentration and elimination rate and urinary excretion following a single oral 500 mg dose of ABOB or repeated administration for 6 days was performed.

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Accession: 068529503

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PMID: 480138

DOI: 10.1248/yakushi1947.99.4_387


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