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Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril



Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril



Biochemical and Biophysical Research Communications 80(4): 849-857



Films of 1,3,4,6-tetrachloro-3a,6a-diphenylglycoluril (conveniently plated in reaction vessels from methylene chloride solution) react rapidly in the solid phase with aqueous mixtures of I- and proteins to yield iodinated proteins. Similarly applied, this reagent brings about iodination of cell membranes [sheep erythrocytes; LPC-1 tumor cells], although, apparently, at a somewhat lesser rate than in iodinations carried out with lactoperoxidase-glucose oxidase. The stability and sparing solubility of this chloroglycoluril in water can account for the minimal damage to proteins and living cells observed in these iodinations; these properties allow for elimination of the reduction step employed at the close of iodinations with soluble chloroamides such as chloramine-T.

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Accession: 068532519

Download citation: RISBibTeXText

PMID: 637870

DOI: 10.1016/0006-291x(78)91322-0


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